By Peter Malcolm Wallis, Brian R. Hammond
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Extra resources for Advances in Giardia Research
The authors propose that the two isolates be given separate species names. One approach to determining the significance of the GiardiaShigella relationship is its study in vitro. Exp. 53:371380. 3, Pergamon Press, Oxford, p 565718. ), Waterborne Transmission of Giardiasis, Environmental Protection Agency 600/979 001, p 217229. 15:2630. 18:126. A) 148:166168. 71:8995. Med. 15:743750. 64:165171. 49:164171. 44:140173. 140:222228. 23:850855. 549556. p 578. Protozool.
Gastroenterology 71:5761. 53:704707. J. 73:723729. Gastroenterology, In press. 921. lamblia trophozoites. These observations suggest that direct cell to parasite contact may play an important role in damaging the host cells. One reported the significance of bile salt deconjugation by the parasite (12) and the other the cytopathogenic effects of Giardia on cell monolayers, in vitro (11). lamblia on HeLa and Vero cells. 30888 (9) were maintained in Diamond's TPS1 (4) complete filter sterilized medium according to the method of Visvesvara (14).
Therefore, Giardia cysts formed in vitro have fulfilled all of the criteria currently being used to measure cyst viability. The development of this in vitro model of the life cycle of Giardia should facilitate the testing of Kochs' postulates, since it will now be possible not only to isolate trophozoites from an animal and grow them in culture, but also to produce cysts in vitro, which could be used to reinfect the same (or possibly a different) type of host. Mention of trade names or material products does not constitute endorsement or recommendation for use.