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BALTZ of CPC with a variety of substituted acetic acids would make very attractive the cloning of the corresponding gene from its source in nature into C. acremonium. If a "precursable cephalosporin fermentation" could be developed via recombinant DNA, this technology might be extended by cloning genes from streptomycetes which are capable of producing 7-o-methyl-cephalosporins. If suitable substrate specificities of the key hydroxylases and methylases exist, expression of the streptomycete genes in C.
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