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Microbiol. 77, 187 (1973). Bibb, M. , Ward, J. M. and Hopwood, D. , Nature 274, 388 (1978). Stewart, G. , J. Amer. Soc. Brew. Chem. 36, 175 (1978). , Yamanaka, S. , J. Ferm. Tech. 56, 539 (1978). Ball, C , Lawrence, A. J. , Butler, J. M. and Morrison, K. , Eur. J. Appl. Microbiol. Biotech. 5, 95 (1978). Das, A. , Can. J. Microbiol. 24, 622 (1978). Thorbek, L. , J. Appl. Bact. 37, 549 (1974). Shcherbakova, E. , Rezvaya, M. , L'vova, E. B. and Movchan, Y. , Mikrobiologiya 47, 91 (1978). 40 96. 97.

BALTZ of CPC with a variety of substituted acetic acids would make very attractive the cloning of the corresponding gene from its source in nature into C. acremonium. If a "precursable cephalosporin fermentation" could be developed via recombinant DNA, this technology might be extended by cloning genes from streptomycetes which are capable of producing 7-o-methyl-cephalosporins. If suitable substrate specificities of the key hydroxylases and methylases exist, expression of the streptomycete genes in C.

Shaw, W. W. and Hopwood, D. , J. Gen. Microbiol. 94, 159 (1976). Freeman, R. , Bibb, M. J. and Hopwood, D. , J. Gen. Microbiol. 98, 453 (1977). , Micheli, M. , Mol. Gen. Genet. 164, 99 (1978). Wright, H. M. and Hopwood, D. , J. Gen. Microbiol. 102, 417 (1977). 2. GENETICS OF INDUSTRIAL MICROORGANISMS 169. 170. 171. 172. 173. 174. 175. 176. 177. 178. 179. 180. 181. 182. 183. 184. 185. 186. 187. 188. 189. 190. 191. 192. 43 Freeman, R. F. and Hopwood, D. , J. Gen. Microbiol. 106, 371 (1978). , Proc.

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