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K a l l e n b a c h and M a (1968) o b s e r v e d that, after readdition of 40 SCOTT WINSTON AND NOBORU SUEOKA t h y m i n e to thymine-starved Β. subtilis cells, the ade/met ratio increased only t o 3 , r a t h e r than to the e x p e c t e d value of 4. T h e y interpreted t h e results to m e a n that p r e m a t u r e initiation o c c u r r e d on only o n e of t w o possible d a u g h t e r c h r o m o s o m e s , which would be a s y m m e t r i c . K a u f m a n (1976) investigated the effect of thymine starvation on germinating spores and exponentially growing cells using density transfer t e c h n i q u e s .
Bacteriol. 139, 6 6 8 - 6 7 0 . 68. Higerd, T. B . (1977). J. Bacteriol. 129, 9 7 3 - 9 7 7 . 69. Higerd, T. B . , H o c h , J. Α . , and Spizizen, J. (1972). J. Bacteriol. 1026-1028. 70. H o c h , J. Α . , and Anagnostopoulos, C. (1970). J. Bacteriol. 103, 2 9 5 - 3 0 1 . 71. H o c h , J. Α . , and Coukoulis, J. H. (1978). J. Bacteriol. 133, 2 6 5 - 2 6 9 . 72. H o c h , J. Α . , and M a t h e w s , J. (1972). In "Spores V " (H. O. Halvorson, R. H a n s o n , and L. L . Campbell, e d s . ) , pp.
Bacteriol. 139, 3 0 8 - 3 1 0 . 165. Siccardi, A . , Fortunata, Α . , and Mazza, G. (1976). J. Bacteriol. 138, 1 7 4 - 1 8 1 . 166. Siegel, E . C , and Marmur, J. (1969). J. Virol. 4, 6 1 0 - 6 1 8 . 167. Smith, D . Α . , Moir, Α . , and S a m m o n s , R. (1978). In "Spores V I I " (G. Chambliss and J. C. Vary, e d s . ) , pp. 158-163. American Society for Microbiology, Washington, D . C. 168. , and Paress, P. (1978). / . Bacteriol. 135, 1101-1117. 169. , and Smith, H. (1973). J. Bacteriol.