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By J. F. Theis, C. S. Newlon (auth.), Prof. Dr. Robert Brambl, Prof. Dr. George A. Marzluf (eds.)

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Aim discovery is a box that has existed for a number of years yet is so vivid at the present time as a result contemporary growth in our knowing of the molecular mechanisms of many human illnesses and the technical advances in aim identity and validation. extra refined gene profiling applied sciences, corresponding to DNA microarrays and serial research of gene expression, let speedy id of lead objectives.

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G. Biogenesis . . . . . . . . . . . . . . . .. H. Regulation. . . . . . . . . . . . . . . IV. PMA2: an Alternative Proton Pump ......... V. PMRl: a Putative Golgi-Based Ca2+ATPase in Saccharomyces cerevisiae . . . . VI. PMR2/ENA: a Putative Na+ Efflux System in Saccharomyces cerevisiae . . . . . . . . VII. PMCl: a Vacuolar Ca2+ Pump in Saccharomyces cerevisiae . . . . . . . . VIII. CTA3: an Endomembrane Ca2+ Pump in Schizosaccharomyces pombe .

Mol Cell Biol13:391-398 Deshpande AM, Newlon CS (1992) The ARS consensus sequence is required for chromosomal origin function in Saccharomyces cerevisiae. Mol Cell Biol 12:43054313 Diffiey JFX (1992) Global regulators of chromosome function in yeast. Antonie Leeuwenhoek 62:25-33 Diffiey JFX, Coeker JH (1992) Protein-DNA interactions at a yeast replication origin. Nature (London) 357: 169-172 Diffiey JFX, Stillman B (1988) Purification of a yeast protein that binds to origins of DNA replication and a transcriptional sileneer.

1988) that encodes a transcription factor that has been demonstrated to act in combination with four other regulatory pro teins and possibly also independently to modulate the transcription of diverse genes (reviewed by Kuo and Grayhack 1994). The DNA-binding domain of Mcm1p shows homology to that of human serum response factor (Norman et al. 1988; Passmore et al. 1989). In addition to its clear role in gene regulation, genetic evidence also implicates MCMI in DNA replication. In addition to the plasmid maintenance defect that was the basis of its initial identification, a temperature-sensitive mutation in MCMI causes temperature-dependent accumulation of large budded cells with a single nucleus, many of which arrest with a partially replicated genome.

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