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By Yu. M. Torchinsky, T. T. Berezov (auth.), Dr. Timo K. Korpela, Prof. Philipp Christen (eds.)

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Additional info for Biochemistry of Vitamin B6 : Proceedings of the 7th International Congress on Chemical and Biological Aspects of Vitamin B6 Catalysis, held in Turku, Finland, June 22–26, 1987

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It developed that When we went out on a fishing boat, Braunstein Prof. knew the common names in several languages of the various fish that we caught. His interest in language facilitated his equally deep interest in the people and my This became evident the cultures of different countries. contacts with him at meetings and during sightseeing tours in in such places as Switzerland, Scandinavia, Italy, Greece, the Soviet Union, Vienna, France, Germany and New York. delightful was hospitality presented Braunstein and with his 6 I will never On arrival in Moscow.

Hence, the main source of glutamic acid in their tissues must be glutamate contained in food or synthesized by transamination of other amino acids with 2-oxoglutarate. The relative importance of different pathways of amino acid 8 metabolism is not conclusively established - their significance varies in living organisms belonging to different classes and in the organs of a single organism. Generally accepted is the fundamental concept of Braunstein that transamination reactions play a crucial role in the biosynthesis and catabolism of most amino acids and in the interrelations between the metabolic pathways of amino acids and those of carbohydrates and fats (Braunstein, 1939a, 1947, 1957).

Coli strain JM105 containing pmAspAtpKK233-2 (A) or pKK233-2 (8) were grown for 4 hours and the proteins extracted for SDS-PAGE (Sheffield et aI, 1986). After the transfer, pmAspAT was detected with anti-mAspAT goat serum and a biotinylated secondary antibody, peroxidase, avidin system (Vector Laboratories). 38 containing pmAspAT-pKK233-2 and not in the control. This polypeptide is consistent with in vivo synthesized pmAspAT. , 1985) are almost identical. , 1985) is seven residues shorter, but the first six and the last eight residues are almost identical to that of pig and mouse.

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