By Willis A. Wood, Scott T. Kellogg
This quantity covers cellulose and hemicellulose and contains confirmed and reproducible equipment for study with regards to the conversion of carbohydrate polymers to usable monomeric devices. Sections at the guidance of biomass fabrics and of substrates are integrated, as are sections on analytical equipment and at the purification and assay of enzymes
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Additional resources for Biomass Part A: Cellulose and Hemicellulose
Another group of solvents capable of dissolving cellulose is the iron-tartaric acid-alkali metal complex. 5 Also known as EWNN and FeTNa, the complex contains an iron : tartaric acid : alkali mole ratio of 1 : 3 : 6 plus 2-3 N NaOH. i G. Jayme and F. Lang, Methods Carbohydr. Chem. III, 75 (1963). 2 E. L. Lovell, Ind. Eng. Chem. 16, 683 (1944). 3 V. H. Krassig and E. Siefert, Makrornol. Chem. 14, 1 (1954). 4 A. A. Strepikheev, I. L. Knunyants, N. S. Nikolaeva, and E. M. Mogilevsky, Bull. Acad. Sci.
This can best be done by suspending the gelatinous mass in the bicarbonate solution overnight. Treatment with a Waring blender is usually necessary to obtain a usable cellulose suspension which is free from acid. Lumps of partially swollen cellulose can be removed by decantation using large measuring cylinders to provide long sedimentation times. HaPO4-swollen cellulose prepared from cotton has a degree of polymerization of approximately 2000: when prepared from Avicel the chain length is approximately 100.
This is especially true for samples containing noncellulosic material. Dissolution time can be decreased by prewetting the sample with a few drops of a nonionic surfactant, such as 1% Triton X-100 (Rohm  CELLULASESUBSTRATES 19 & Haas, Philadelphia, PA) prior to dissolution. Solubilization can be further speeded by milling the cellulose prior to wetting with the surfactant. Cellulose/cadoxen solutions may be stored in the freezer and are stable for up to 4 months, after which the solutions slowly turn cloudy.