By Sidney Fleischer, Becca Fleischer (Eds.)
The seriously acclaimed laboratory common, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. seeing that 1955, every one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - actually a necessary book for researchers in all fields of existence sciences
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Extra info for Biomembranes Part T
These outputs are attached to an x - y recorder (Hewlett-Packard 7035B, or Sefram XIP, for example). Detailed manuals are available from the Technicon company. Diluents The two essential characteristics of the diluent used in ektacytometry are (1) a refractive index different from that of the cells or ghosts, so that a diffraction image is generated, and (2) a viscosity greater than the internal viscosity of the particles. Red cells in shear flow behave as liquid droplets, and will not deform unless external viscosity exceeds internal (if this condition is not met, the cells simply tumble).
Next, experimental procedures and computational algorithms for determination of membrane mechanical properties will be given for the normal behavior of the cell membrane as a "viscoelastic" solid material. This will be followed by more complicated rheology of the membrane in lysis and in shear failure ("creep" and plastic flow). Red Cell Area Dilation Because of the osmotic strength of the cytoplasmic contents, red cell area can be easily dilated by application of large micropipet suction pressures to osmotically preswollen red cells as illustrated in Fig.
The red cell was osmotically preswollen before aspiration by the pipet. Because the cell volume is essentially fixed, displacement of the cell into the micropipet requires surface area dilation. Aspiration pressures are in the range of 104-105 dyn/cm 2 for the pipet caliber of 2 x 10-4 cm. proportionally, -- PRp/2(I - Rp/Ro) Here, P is the pipet suction pressure; Rp is the pipet inner radius; and Ro is the radius of the outer spherical segment of the red cell. 1 M, less than 20% of the displacement of the aspirated length into the pipet is due to filtration of water from the cell interior.