Download Calcium Signaling Protocols by Alec W. M. Simpson (auth.), David G. Lambert, Richard D. PDF

By Alec W. M. Simpson (auth.), David G. Lambert, Richard D. Rainbow (eds.)

The law of intracellular Ca2+ has persisted to be a robust region of analysis because the ebook of the 1st and moment variants of Calcium Signaling Protocols, and the advancements within the box have additionally, certainly, endured. With the 3rd variation, professional individuals discover a number of the fascinating new molecular innovations that experience either enabled new experiences of intracellular Ca2+ rules and supplied a lot new details on strategies. created from 5 major part, the e-book covers theoretical and intensely easy suspension-based fluorimetric assays, expert size structures, size of channel job, dimension of shop unlock, in addition to expert size strategies which come with exact probes, utilizing G-protein chimeras to strength Ca2+ signalling for screening, and genetically encoded sensors. Written within the hugely winning Methods in Molecular Biology™ sequence structure, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, without problems reproducible laboratory protocols, and pointers on troubleshooting and keeping off recognized pitfalls.

Updated and obtainable, Calcium Signaling Protocols, 3rd Edition might be a invaluable reference for all these considering a movement into the examine of intracellular Ca2+.

Show description

Read or Download Calcium Signaling Protocols PDF

Similar biochemistry books

Target Discovery and Validation Reviews and Protocols: Volume 1, Emerging Strategies for Targets and Biomarker Discovery

Goal discovery is a box that has existed for numerous years yet is so bright this present day as a result of contemporary development in our figuring out of the molecular mechanisms of many human ailments and the technical advances in aim identity and validation. extra refined gene profiling applied sciences, resembling DNA microarrays and serial research of gene expression, let fast identity of lead goals.

Extra info for Calcium Signaling Protocols

Example text

26. 27. 28. 29. 30. 31. 32. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations in fura-2-loaded human platelets. FEBS Lett 222:144–148 Morris SJ et al (1994) Rapid simultaneous estimation of intracellular calcium and pH. Methods Cell Biol 40:183–220 Martinez-Zaguilan R, Parnami G, Lynch RM (1996) Selection of fluorescent ion indicators for simultaneous measurements of pH and Ca2+. Cell Calcium 19:337–349 Belan PV et al (1996) A new technique for assessing the microscopic distribution of cellular calcium exit sites.

Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations in fura-2-loaded human platelets. FEBS Lett 222:144–148 Morris SJ et al (1994) Rapid simultaneous estimation of intracellular calcium and pH. Methods Cell Biol 40:183–220 Martinez-Zaguilan R, Parnami G, Lynch RM (1996) Selection of fluorescent ion indicators for simultaneous measurements of pH and Ca2+. Cell Calcium 19:337–349 Belan PV et al (1996) A new technique for assessing the microscopic distribution of cellular calcium exit sites.

Proc Natl Acad Sci USA 98:3197–3202 103. Nakai J, Ohkura O, Imoto K (2001) A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein. Nat Biotechnol 19:137–141 104. Robert V et al (2001) Beat-to-beat oscillations of mitochondrial [Ca2+] in cardiac cells. EMBO J 20:4998–5007 105. Nagai T et al (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc Natl Acad Sci USA 101:10554–10559 106. Palmer AE et al (2004) Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor.

Download PDF sample

Rated 4.96 of 5 – based on 29 votes